DSIP protocol · PepGuide

Check your units. The units in this table assume 2 mg/mL or 3.33 mg/mL, but the guide's recommended mix makes 5 mg/mL. If you mix it that way, your units will be different. Use the calculator to get the right draw for your vial.

Standard

Weeks 1–2

10u (2 mg/mL) / 6.0u (3.33 mg/mL)

200 mcg · Nightly

Start low and assess how your body responds before increasing.

Weeks 3–8

10–25u (2 mg/mL) / 6.0–15.0u (3.33 mg/mL)

200–500 mcg · Nightly

Effective range.

Off

2–4 weeks

Essential break between cycles. Skipping leads to tolerance and diminishing returns.

Phase	How often	Dose	Units	Notes
Weeks 1–2	Nightly	200 mcg	10u (2 mg/mL) / 6.0u (3.33 mg/mL)	Start low and assess how your body responds before increasing.
Weeks 3–8	Nightly	200–500 mcg	10–25u (2 mg/mL) / 6.0–15.0u (3.33 mg/mL)	Effective range.
Off	2–4 weeks	–	–	Essential break between cycles. Skipping leads to tolerance and diminishing returns.

Units columns in the source are labelled for 2 mg/mL and 3.33 mg/mL, not the 5 mg/mL reconstitution above.

Cycle & timing

Cycle: 4–8 weeks on, 2–4 weeks off.

Timing: Pre-bed nightly, 15–30 min before sleep.

Where: SubQ abdomen.

What to expect

Deeper, more restorative sleep typically within the first few doses. Reduced overnight cortisol. Vivid dreams in some users.

How much to buy

For 8-wk @ 500 mcg × 7/wk:

12

× 2mg vials

5

× 5mg vials

3

× 10mg vials

Men & women

Unisex sleep peptide, same protocol for men and women, no cycle effect.

Dilution calculator

Set to the guide's typical mix for DSIP. Change anything to see where to draw.

Vial size

BAC water added

Dose you want

Draw to

4u

Volume

0.04 mL

Strength

5 mg/mL

Doses per vial

25

Assumes a U-100 insulin syringe (100 units = 1 mL). Double-check against the compound's page before you draw.

Recommended mixes

Vial	Water	Strength
2 mg	0.4 mL	5 mg/mL
5 mg	1 mL	5 mg/mL
10 mg	2 mL	5 mg/mL

2 mg: 1 unit = 0.05 mg (50 mcg)

Storage

PowderFreeze-dried: -4°F.
MixedReconstituted: 36–46°F, use within 4 weeks. Protect from light.

Don't use if: Pregnancy · Lactation · Active or history of cancer (theoretical GH-related concern)

Stop if you notice

Excessive sedation
Vivid dreams disturbing sleep
Persistent daytime grogginess

Side effects

Sedation (expected), headache (occasional), vivid dreams.

Drug interactions

CNS depressants (benzodiazepines, Z-drugs, barbiturates, opioids, alcohol): additive sedation; avoid same-night combo or reduce dose. Other sleep agents (melatonin, Epithalon): redundant rather than additive; pick one per cycle. Sedating antidepressants (mirtazapine, trazodone, doxepin, amitriptyline): additive sedation; coordinate timing. Stimulants (caffeine, ADHD meds): mechanistically opposed; not harmful but blunts effect. Antihypertensives: theoretical additive nocturnal BP drop, monitor if combining at high doses.

Labs

Compound-specific: CBC + CMP baseline; no compound-specific markers routinely required

What these labs mean →

Warnings

Limited human data. PCAC REJECTED it (as "emideltide") from the 503A bulks list, 6–7 (Jul 2026); the only one of seven peptides voted down.

Patient education

Aseptic technique: alcohol swab the vial septum and the injection site, use a single-use sterile needle for every injection, never share vials or needles between people. Sharps disposal in an FDA-cleared sharps container, never household trash. Cold chain: if a refrigerated peptide reaches room temperature for more than the labeled stability window, discard it. Mark the date of first puncture on every multi-dose vial. Inspect every vial before injection: clear solution (unless labeled otherwise), no particulates, no cloudiness, no color change. Rotate injection sites to avoid lipoatrophy, induration, and infection. Carry a record of what you're injecting if you need emergency care.

These apply on top of the universal safety rules.

Pairs well with

Melatonin

sleep architecture

Melatonin

Glycine

deep-sleep amplification

GHK-Cu

recovery during sleep

GHK-Cu

Avoid with

Active or history of cancer

theoretical GH-related concern

Pregnancy / nursing

Can DSIP + Melatonin share a syringe?

Give it its own pin. The guide says this one goes by itself.

What it does

Evidence: minimal human data; no dosing RCT.

Delta Sleep-Inducing Peptide, promotes slow-wave (delta) sleep, reduces stress hormones, and regulates circadian rhythm. Modulates CRH. May regulate pineal.

Approved drug

Approved insomnia drugs: the orexin blockers suvorexant (Belsomra), lemborexant (Dayvigo) and daridorexant (Quviviq); low-dose doxepin (Silenor); ramelteon (Rozerem); and zolpidem.

References

researchdosing peptidedosages PubMed thepeptidereport peptides.id

From the guide, page 30.