Glutathione protocol

Standard

Weeks 1–2

20u

100 mg · Mon–Fri upper glute

Tolerance check at 100 mg. 5×/week = 500 mg/week. Watch for garlic taste, flushing, or injection-site sting.

Weeks 3–8

40u

200 mg · Mon/Wed/Fri upper glute

Working dose ceiling (PeptideDosages canonical). 3×/week = 600 mg/week. Matches Empower/AgelessRx clinic 200 mg dose. Hold at 100 mg if 200 mg poorly tolerated.

Maintenance

40u

200 mg · 1–2×/week

Ongoing antioxidant support. Drop to once-weekly after the 8-week loading + working phase if benefit holds.

Off

2–4 weeks

Essential break. Skipping leads to tolerance and diminishing returns.

Phase	How often	Dose	Units	Notes
Weeks 1–2	Mon–Fri upper glute	100 mg	20u	Tolerance check at 100 mg. 5×/week = 500 mg/week. Watch for garlic taste, flushing, or injection-site sting.
Weeks 3–8	Mon/Wed/Fri upper glute	200 mg	40u	Working dose ceiling (PeptideDosages canonical). 3×/week = 600 mg/week. Matches Empower/AgelessRx clinic 200 mg dose. Hold at 100 mg if 200 mg poorly tolerated.
Maintenance	1–2×/week	200 mg	40u	Ongoing antioxidant support. Drop to once-weekly after the 8-week loading + working phase if benefit holds.
Off	2–4 weeks	–	–	Essential break. Skipping leads to tolerance and diminishing returns.

Cycle & timing

Cycle: 8 weeks on per the protocol (weeks 1–8), then 1–2×/week maintenance; 2–4 weeks off. Max: 12 weeks on / 4–8 weeks off. Hepatic methylation cycling supports best results with breaks.

Timing: Mon–Fri for weeks 1–2, then Mon/Wed/Fri (per the protocol table), reconstitute fresh; oxidizes within hours.

Where: Upper outer glute preferred. Abdomen acceptable.

What to expect

Energy improvement and reduced oxidative fatigue within 2–4 weeks. Skin brightening reported by many users. Must reconstitute fresh, oxidises within hours of reconstitution.

How much to buy

For 8-wk @ 200 mg × 3/wk:

8

× 600mg vials

4

× 1500mg vials

Men & women

Unisex antioxidant, same protocol for men and women, no cycle effect. Confirm with a prescriber in pregnancy.

Dilution calculator

Set to the guide's typical mix for Glutathione. Change anything to see where to draw.

Vial size

BAC water added

Dose you want

Draw to

40u

Volume

0.40 mL

Strength

500 mg/mL

Doses per vial

3

Assumes a U-100 insulin syringe (100 units = 1 mL). Double-check against the compound's page before you draw.

Recommended mixes

Vial	Water	Strength
600 mg	1.2 mL	500 mg/mL
1500 mg	3 mL	500 mg/mL

600 mg: 1 unit = 5.00 mg

Storage

PowderFreeze-dried: -4°F (stable long-term).
MixedReconstituted: use fresh, do not store for weeks. Glutathione oxidizes to GSSG within hours at room temperature or once exposed to air/light; kept cold (36–46°F) and dark in the sealed amber vial it lasts only a few days.
Toss itDiscard the moment it yellows (that's oxidation). Never room temperature; never freeze (ice crystals denature the tripeptide).

Don't use if: Sulfa allergy (theoretical, reaction-prone individuals) · Concurrent chemotherapy without oncology supervision

Stop if you notice

Rash, hives, or sulfhydryl-type allergic reaction
Severe injection-site irritation

Side effects

Upper glute sting (brief). Flushing or garlic taste within 30 min. Reconstitute fresh only.

Drug interactions

Chemotherapy (cisplatin, doxorubicin, cyclophosphamide and others): glutathione may reduce both toxicity AND efficacy; do NOT combine outside an oncologist-supervised protocol. Acetaminophen overdose treatment (N-acetylcysteine pathway): glutathione is the protected metabolite, supplemental IV/SubQ glutathione may augment NAC therapy but is not a substitute. Warfarin: theoretical mild potentiation via reduced oxidative stress on coagulation factors; monitor INR if combining chronically. Sulfa drugs (sulfonamide antibiotics, sulfasalazine, some diuretics): increased reaction risk in sulfa-allergic patients given the sulfhydryl group. Methylating agents and SAMe: complementary methylation/redox support; theoretical synergy, no human interaction data.

Labs

Compound-specific: CBC + CMP baseline; no compound-specific markers routinely required

What these labs mean →

Warnings

Must be reconstituted fresh; oxidizes within hours. FDA has flagged compounded IV glutathione for skin-lightening use as an unproven indication with serious adverse event reports.

Patient education

Reconstitute immediately before use; discard unused, glutathione oxidizes within hours and is no longer active. Do not use during active cancer treatment without oncology coordination. Skin-lightening use is unproven and the FDA has issued safety warnings about compounded IV products marketed for that purpose.

These apply on top of the universal safety rules.

Pairs well with

NAD+

cellular redox stack

NAD+

GHK-Cu

additive antioxidant

GHK-Cu

Vitamin C

recycle glutathione

BPC-157

gut + systemic recovery

BPC-157

MOTS-c

mitochondrial

MOTS-c

Avoid with

Sulfa allergy

theoretical

Concurrent chemotherapy

may interfere, physician supervision

Can Glutathione + NAD+ share a syringe?

Give it its own pin. Glutathione oxidizes within hours. Own pin, and never with copper or vitamin C.

Changes from the guide

The guide contradicted itself or had a slip here, so this page differs from the PDF:

The guide's cycle line said 4 on / 4 off and timing said daily, but its protocol runs 8 weeks, Mon–Fri then Mon/Wed/Fri. Cycle and timing now follow the protocol table.

What it does

Master antioxidant tripeptide central to oxidative stress defence, liver detox, and immune function. Directly neutralises ROS, regenerates vitamins C and E, supports liver Phase II detox, and maintains mitochondrial integrity.

Fresh-dose note

A reconstituted vial covers ~3 doses but would sit refrigerated across the week, if you want each dose truly fresh, reconstitute a smaller vial (or add less BAC water for fewer doses) more often rather than holding one vial for several days.

References

researchdosing peptidedosages DailyMed PubMed

From the guide, page 38.