PE-22-28 protocol · PepGuide

Check your units. The units in this table assume 2 mg/mL, but the guide's recommended mix makes 5 mg/mL. If you mix it that way, your units will be different. Use the calculator to get the right draw for your vial.

Research-derived

Weeks 1–4

12.5–25u

250–500 mcg · Daily

SubQ or intranasal. Research-derived range; no validated human dose.

Off

2–4 weeks

Cycle; neurogenic effects build over the on-period.

Phase	How often	Dose	Units	Notes
Weeks 1–4	Daily	250–500 mcg	12.5–25u	SubQ or intranasal. Research-derived range; no validated human dose.
Off	2–4 weeks	–	–	Cycle; neurogenic effects build over the on-period.

Units column header in the source reads "Units (2 mg/mL)". Caution: entirely preclinical, no human trials, safety data, or approved dose. Doses are extrapolated from rodent studies (0.1–1 mg/kg). Included for completeness. Do not use in place of evaluated treatment for depression.

What to expect

Unknown in humans. Animal data suggest rapid mood/neurogenesis effects, not demonstrated in people.

How much to buy

For 4-wk @ 250 mcg/day, research-derived:

2

× 5mg vials

1

× 10mg vials

Men & women

Unisex experimental antidepressant peptide, animal data only, no sex-specific dosing. Avoid in pregnancy.

Dilution calculator

Set to the guide's typical mix for PE-22-28. Change anything to see where to draw.

Vial size

BAC water added

Dose you want

Draw to

5u

Volume

0.05 mL

Strength

5 mg/mL

Doses per vial

20

Assumes a U-100 insulin syringe (100 units = 1 mL). Double-check against the compound's page before you draw.

Recommended mixes

Vial	Water	Strength
5 mg	1 mL	5 mg/mL
10 mg	2 mL	5 mg/mL

5 mg: 1 unit = 0.05 mg (50 mcg)

Storage

PowderFreeze-dried: -4°F.
MixedReconstituted: 36–46°F, up to 4 weeks.

Don't use if: Pregnancy and lactation · Use as a substitute for evaluated psychiatric care · Hypersensitivity to the peptide

Stop if you notice

Worsening mood, agitation, or any manic symptoms
Any unexpected systemic reaction
Suicidal thoughts, seek immediate care

Side effects

Unknown in humans. Theoretically favorable (spadin lacks SSRI-type side effects in animals), but unverified.

Drug interactions

Other antidepressants / serotonergic agents: unknown combined effect. Uncharacterized in humans.

Labs

None established; psychiatric monitoring if used for mood

What these labs mean →

Warnings

No human safety, efficacy, or dosing data; entirely preclinical. Mood disorders require proper evaluation, do not self-treat depression with a research peptide. Bipolar/mania risk with any antidepressant mechanism, unknown here. Quality varies, request a COA.

Patient education

Promising mechanism but zero human data, treat as experimental. Not a replacement for evaluated treatment of depression. If you have a mood disorder, work with a clinician. Seek immediate help for worsening mood or suicidal thoughts.

These apply on top of the universal safety rules.

Pairs well with

Semax / Selank

mood + cognition

Semax Selank

P21

neurogenesis. All combinations unproven in humans

P21 (P021)

Avoid with

Replacing prescribed antidepressant therapy

Bipolar disorder without supervision

Pregnancy

Can PE-22-28 + Semax share a syringe?

Can share a syringe. Both are water-based. Combine them if you dose on the same schedule and site.

What it does

No completed human trials, no validated human dose or safety data.

PE-22-28 is an optimized analog of spadin that blocks the TREK-1 potassium channel, producing rapid antidepressant-like effects and increased hippocampal neurogenesis/synaptogenesis within ~4 days in rodents. Longer-acting than spadin (~23 h vs ~7 h).

References

PubMed researchdosing peptidedosages

From the guide, page 73.