Wolverine Blend protocol

Standard

Weeks 1–2

5u

500 mcg · Daily

Loading: assess tolerance. Daily because BPC-157’s serum half-life is short (~30 min); TB-500 rides along in the pre-mixed vial (weekly as a standalone).

Weeks 3–4 (Aggressive)

7.5u

750 mcg · Daily

Step up only if Weeks 1–2 well tolerated. Half-unit step is optional – many users go directly to 1 mg.

Weeks 5–10

10u

1 mg · Daily AM

Working dose. Once daily preferred; optional AM/PM split (5u + 5u) if you find injection volume uncomfortable.

Off

2–4 weeks

Standard washout. Re-cycle if continued tissue work needed.

Phase	How often	Dose	Units	Notes
Weeks 1–2	Daily	500 mcg	5u	Loading: assess tolerance. Daily because BPC-157’s serum half-life is short (~30 min); TB-500 rides along in the pre-mixed vial (weekly as a standalone).
Weeks 3–4 (Aggressive)	Daily	750 mcg	7.5u	Step up only if Weeks 1–2 well tolerated. Half-unit step is optional – many users go directly to 1 mg.
Weeks 5–10	Daily AM	1 mg	10u	Working dose. Once daily preferred; optional AM/PM split (5u + 5u) if you find injection volume uncomfortable.
Off	2–4 weeks	–	–	Standard washout. Re-cycle if continued tissue work needed.

Aggressive

10u

1 mg ×2 · Twice daily (AM + PM split)

For acute injury, post-surgical recovery, or a stalled response. 1 mg blend twice daily = 1 mg of each component daily. 4–6 weeks, then taper to the daily working dose.

Phase	How often	Dose	Units	Notes
Aggressive	Twice daily (AM + PM split)	1 mg ×2	10u	For acute injury, post-surgical recovery, or a stalled response. 1 mg blend twice daily = 1 mg of each component daily. 4–6 weeks, then taper to the daily working dose.

Aggressive (twice daily, acute injury): 4–6 weeks split-dose / 4 weeks off, then back to daily working dose.

Cycle & timing

Cycle: 8–10 weeks on / 2–4 weeks off (daily microdose). Aggressive (twice daily, acute injury): 4–6 weeks split-dose / 4 weeks off, then back to daily working dose.

Timing: Daily, anytime; Wolverine Blend; food doesn’t affect.

Where: SubQ near injury site. Rotate.

What to expect

Faster recovery than either alone. Pain reduction within days, structural repair over 4–12 weeks. Systemic anti-inflammatory effect complements localised healing.

How much to buy

For 10-wk @ 1 mg × 7/wk:

6

× 10mg vials

3

× 20mg vials

2

× 30mg vials

Men & women

Unisex (BPC-157 + TB-500 recovery), same protocol for men and women, no cycle effect. Avoid in pregnancy.

Dilution calculator

Set to the guide's typical mix for Wolverine Blend. Change anything to see where to draw.

Vial size

BAC water added

Dose you want

Draw to

10u

Volume

0.10 mL

Strength

10 mg/mL

Doses per vial

10

Assumes a U-100 insulin syringe (100 units = 1 mL). Double-check against the compound's page before you draw.

Recommended mixes

Vial	Water	Strength
10 mg	1 mL	10 mg/mL
20 mg	2 mL	10 mg/mL
30 mg	3 mL	10 mg/mL

10 mg: 1 unit = 0.10 mg

Storage

PowderFreeze-dried: -4°F.
MixedReconstituted: 36–46°F, up to 8 weeks with continuous refrigeration.

Don't use if: Active malignancy of any kind (TB-500 angiogenic; BPC-157 growth-promoting) · Active bleeding or recent hemorrhagic stroke (TB-500 vascular) · Pregnancy, lactation

Stop if you notice

New mass or unexpected tissue growth at any injection site
Persistent injection-site reactions >7 days (redness, induration, abscess)
New unexplained GI symptoms or bleeding
Vascular changes, new varicosities, persistent skin discoloration

Side effects

Usually minimal. Occasional lightheadedness.

Drug interactions

NSAIDs: don’t blunt the blend (a myth), in animals BPC-157 even protects against NSAID gut/liver damage; still skip during repair (NSAIDs slow healing on their own). Anticoagulants / antiplatelets (warfarin, DOACs, aspirin, clopidogrel): caution with TB-500 vascular effects; monitor INR/anti-Xa if combining. Chemotherapy: do not combine, both promote angiogenesis/tissue growth. Other peptides at same site: rotate sites to reduce local irritation.

Labs

Compound-specific: hsCRP (baseline + end)

What these labs mean →

Warnings

No published human RCTs for the combination. BPC-157: FDA Category 2 (2023); PCAC RECOMMENDED it for the 503A bulks list, 8–6 (Jul 2026; advisory, non-binding). TB-500: theoretical malignancy concern via angiogenesis; no human efficacy trials; also RECOMMENDED by PCAC (8–6, Jul 2026). Vial quality varies, request COA for each lot. Tissue-repair pathways overlap with cancer, any unexplained mass requires evaluation.

Patient education

Two-peptide stack, any single-component contraindication applies. Active cancer is an absolute stop. Both are research peptides without published human RCTs. All use is off-label; quality varies. Local injection near target tissue for orthopedic use; SubQ near abdomen for systemic. Rotate sites. Continuous dosing isn’t supported and may blunt response.

These apply on top of the universal safety rules.

Pairs well with

GHK-Cu

skin + collagen synergy

GHK-Cu

Ipamorelin / CJC No DAC

GH-mediated repair

Ipamorelin CJC-1295 (No DAC)

Tesamorelin

GHRH adjunct

Tesamorelin

KPV

gut anti-inflammatory

KPV

Avoid with

NSAIDs during a repair cycle

ibuprofen/Advil/Aleve slow healing on their own; use Tylenol for pain

Active malignancy

High-dose vitamin C same session

GLOW / KLOW Blend

already contains BPC + TB – would double-dose

GLOW Blend KLOW Blend

Can Wolverine Blend + GHK-Cu share a syringe?

On the fence: keep separate. GHK-Cu is fine freshly drawn, but copper oxidizes sensitive peptides. Never store it mixed, keep it away from vitamin C, and if unsure give it its own pin.

Changes from the guide

The guide contradicted itself or had a slip here, so this page differs from the PDF:

Patient education also gave a different cycle (Cycle: 4–6 wks on / 2–4 wks off). Removed so the page uses the main cycle line: 8–10 weeks on / 2–4 weeks off (daily microdose). Aggressive (twice daily, acute injury): 4–6 weeks split-dose / 4 weeks off, then back to daily working dose.

What it does

Research peptides without published human RCTs; all use is off-label.

Wolverine Blend (BPC-157 + TB-500) – pre-mixed healing blend: BPC-157 (local repair + angiogenesis) + TB-500 (systemic actin modul BPC-157 activates VEGF/NO/GHR pathways for local tissue repair.

Painkillers vs this

Painkillers only mask pain; this blend aims to rebuild the tissue. Skip NSAIDs (ibuprofen, Advil, Aleve, aspirin) during a cycle; they slow healing on their own; if you need pain relief, Tylenol is the better pairing. The old belief that NSAIDs “cancel out” the blend is a myth. Honest caveat: the healing evidence is still mostly from animals, not human trials.

References

researchdosing peptidedosages PubMed thepeptidereport peptides.id

From the guide, page 107.