Humanin protocol · PepGuide

Standard

Weeks 1–6

10–40u

0.5–2 mg · Daily or several×/week

SubQ. Start at the low end. Research/community range, no validated human dose.

Off

2–4 weeks

Cycle 4–8 weeks on / 2–4 weeks off.

Phase	How often	Dose	Units	Notes
Weeks 1–6	Daily or several×/week	0.5–2 mg	10–40u	SubQ. Start at the low end. Research/community range, no validated human dose.
Off	2–4 weeks	–	–	Cycle 4–8 weeks on / 2–4 weeks off.

Units column is for 5 mg/mL.

Cycle & timing

Cycle: 4–8 weeks on / 2–4 weeks off.

Timing: Any time; pairs naturally with MOTS-c as a mitochondrial stack.

Where: SubQ, abdomen. Rotate.

What to expect

Subtle metabolic and cytoprotective support over weeks; a longevity/recovery adjunct rather than an acute-effect peptide.

How much to buy

For 6-wk @ 1 mg/day; fewer if dosed several×/week:

5

× 10mg vials

Men & women

Unisex mitochondrial-derived peptide, no sex-specific dosing; research-stage. Avoid in pregnancy.

Dilution calculator

Set to the guide's typical mix for Humanin. Change anything to see where to draw.

Vial size

BAC water added

Dose you want

Draw to

20u

Volume

0.20 mL

Strength

5 mg/mL

Doses per vial

10

Assumes a U-100 insulin syringe (100 units = 1 mL). Double-check against the compound's page before you draw.

Recommended mixes

Vial	Water	Strength
10 mg	2 mL	5 mg/mL

10 mg: 1 unit = 0.05 mg (50 mcg). A 1 mg dose = 20 units.

Storage

PowderFreeze-dried: -4°F.
MixedReconstituted: 36–46°F, up to 4 weeks.

Don't use if: Active malignancy (anti-apoptotic mechanism, theoretical concern) · Pregnancy and lactation · Hypersensitivity to the peptide

Stop if you notice

New mass or unexplained symptoms
Persistent injection-site reaction
Pregnancy

Side effects

Generally well tolerated in reports, mild injection-site reaction. Human profile not formally characterized.

Drug interactions

No well-characterized interactions. Stacks cleanly with other mitochondrial/longevity peptides.

Labs

Fasting glucose / metabolic panel if used for metabolic goals. Age-appropriate cancer screening if used long-term. Out of range? Rising glucose/HbA1c = blood sugar creeping up (recheck, adjust).

What these labs mean →

Warnings

Anti-apoptotic (pro-survival) signaling, theoretical caution with cancer. Quality varies, request a COA. Effects are subtle and cumulative.

Patient education

A mitochondrial/longevity adjunct, give it weeks, expect subtle effects. Pairs naturally with MOTS-c and NAD+. Request a COA; source quality matters.

These apply on top of the universal safety rules.

Pairs well with

MOTS-c

mitochondrial synergy

MOTS-c

NAD+

cellular energy

NAD+

SS-31

mitochondrial protection

SS-31 (Elamipretide)

Epithalon

longevity

Epithalon

Avoid with

Active malignancy

anti-apoptotic signaling, theoretical

Pregnancy

Can Humanin + MOTS-c share a syringe?

Can share a syringe. Both are water-based. Combine them if you dose on the same schedule and site.

What it does

No formal human clinical dose; community use is extrapolated from animal data.

Humanin is a 24-amino-acid mitochondrial-derived peptide (the analog HNG / S14G-humanin is more potent). It is cytoprotective and anti-apoptotic, improves insulin sensitivity, and protects neurons; originally discovered as a factor shielding cells from Alzheimer's-related death.

Note

No completed human trials, effects are inferred from animal/longevity research.

References

researchdosing peptidedosages PubMed thepeptidereport

From the guide, page 44.