MGF protocol · PepGuide

Check your units. The units in this table assume 1 mg/mL, but the guide's recommended mix makes 2 mg/mL. If you mix it that way, your units will be different. Use the calculator to get the right draw for your vial.

Standard

Training days

20–40u

200–400 mcg · Post-workout

IM directly into the trained muscle within 5–10 min of the last set (half-life is minutes).

Off days

No benefit off training days for native MGF, the stimulus is the workout.

Phase	How often	Dose	Units	Notes
Training days	Post-workout	200–400 mcg	20–40u	IM directly into the trained muscle within 5–10 min of the last set (half-life is minutes).
Off days	–	–	–	No benefit off training days for native MGF, the stimulus is the workout.

Units column header in the source reads "Units (1 mg/mL)". If you want a wider dosing window or systemic effect, use PEG-MGF instead.

Cycle & timing

Cycle: Run during training blocks, 6–8 weeks, then break.

Timing: Immediately post-workout, local to the worked muscle; this is the whole point of native (non-PEG) MGF.

Where: IM, into the specific trained muscle. Rotate within the muscle.

What to expect

Enhanced local recovery and hypertrophy in the trained muscle over a training block. Effects are local and modest, not dramatic.

How much to buy

For 8-wk @ 200 mcg, ~4 training days/wk:

4

× 2mg vials

Men & women

A local IGF-1 splice variant, like the GH axis, women may respond at a slightly lower dose. Efficacy contested; avoid in pregnancy.

Dilution calculator

Set to the guide's typical mix for MGF. Change anything to see where to draw.

Vial size

BAC water added

Dose you want

Draw to

10u

Volume

0.10 mL

Strength

2 mg/mL

Doses per vial

10

Assumes a U-100 insulin syringe (100 units = 1 mL). Double-check against the compound's page before you draw.

Recommended mixes

Vial	Water	Strength
2 mg	1 mL	2 mg/mL

2 mg: 1 unit = 0.02 mg (20 mcg)

Storage

PowderFreeze-dried: -4°F.
MixedReconstituted: 36–46°F, up to 3–4 weeks, away from light.

Don't use if: Active malignancy of any kind (IGF-1 variant, growth-promoting) · Pregnancy and lactation · Hypersensitivity to the peptide

Stop if you notice

New mass or unexplained tissue growth
Persistent injection-site reaction
Pregnancy

Side effects

Usually minimal, local soreness. Theoretical IGF-class concerns at high/systemic exposure.

Drug interactions

No well-characterized interactions. Stacks with GH-axis and repair peptides, mind cumulative IGF signaling.

Labs

IGF-1 if used heavily or stacked with GH-axis peptides. Age-appropriate cancer screening if used long-term. Out of range? If you stack it with GH-axis peptides, IGF-1 above your age range = too much growth signal → back off.

What these labs mean →

Warnings

Efficacy is contested, a rigorous two-lab replication (Fornaro 2014) found the MGF peptide failed to affect muscle-cell proliferation or differentiation, questioning whether it has a real physiological effect. Treat benefits as unproven. IGF-family growth signaling, theoretical tumor-promotion concern. Local, short-acting; benefit is limited to the injected muscle. Limited human data; quality varies, request a COA.

Patient education

Inject locally and immediately post-workout, the short half-life makes timing everything. Effects are local and modest; pair with PEG-MGF for a wider window. Avoid with active cancer due to growth signaling. Report new lumps or persistent injection-site reactions.

These apply on top of the universal safety rules.

Pairs well with

PEG-MGF

systemic complement

PEG-MGF

IGF-1 LR3

satellite-cell synergy

IGF-1 LR3

CJC-1295 + Ipamorelin

GH-axis recovery

CJC-1295 (No DAC) + Ipamorelin

Wolverine Blend

BPC-157 + TB-500

Wolverine Blend

Avoid with

Active malignancy

IGF-class growth signaling

Injecting non-locally for native MGF

wastes it

Can MGF + PEG-MGF share a syringe?

Compatible, but check the timing. IGF-1 / MGF / HGH are water-based and compatible. They're often timed post-workout, so you may separate them for timing, not safety.

What it does

MGF (IGF-1Ec) activates muscle satellite cells, driving proliferation and local repair/hypertrophy after mechanical damage. Its half-life is only ~5–7 minutes, so it must be injected locally into the target muscle within minutes of the last set. For systemic/longer action, use the pegylated version (PEG-MGF).

References

researchdosing peptidedosages PubMed thepeptidereport

From the guide, page 66.