NAD Buffer protocol

Check your units. The units in this table assume 100 mg/mL, but the guide's recommended mix makes 500 mg/mL. If you mix it that way, your units will be different. Use the calculator to get the right draw for your vial.

Standard

Weeks 1–2 (ramp)

25–50u

25–50 mg · 2–3×/week

SubQ, injected SLOWLY. Buffering helps, but slow is still key.

Weeks 3+

50–100u

50–100 mg · 2–3×/week

Titrate up by tolerance. Still inject slowly to minimize flushing.

Phase	How often	Dose	Units	Notes
Weeks 1–2 (ramp)	2–3×/week	25–50 mg	25–50u	SubQ, injected SLOWLY. Buffering helps, but slow is still key.
Weeks 3+	2–3×/week	50–100 mg	50–100u	Titrate up by tolerance. Still inject slowly to minimize flushing.

Units column header in the source reads "Units (100 mg/mL)". Key: the buffer reduces discomfort, but slow injection remains the main way to avoid the flush/chest-tightness; never push a bolus.

Cycle & timing

Cycle: Ongoing or 8–12 week blocks.

Timing: Daytime (can be energizing).

Where: SubQ, abdomen, injected slowly. Rotate.

What to expect

Same energy/recovery/longevity profile as NAD+, with a more comfortable injection. Effects build over weeks.

How much to buy

For 8-wk @ 100 mg × 3/wk:

3

× 500mg vials

2

× 1000mg vials

Men & women

N/A, a buffer / reagent for reconstitution, not a dosed compound.

Dilution calculator

Set to the guide's typical mix for NAD Buffer. Change anything to see where to draw.

Vial size

BAC water added

Dose you want

Draw to

20u

Volume

0.20 mL

Strength

500 mg/mL

Doses per vial

5

Assumes a U-100 insulin syringe (100 units = 1 mL). Double-check against the compound's page before you draw.

Recommended mixes

Vial	Water	Strength
500 mg	1 mL	500 mg/mL
1000 mg	2 mL	500 mg/mL

500 mg: 1 unit = 5 mg

Storage

PowderFreeze-dried: -4°F.
MixedReconstituted: 36–46°F, up to 4 weeks. Protect from light.

Don't use if: Active malignancy (NAD-pathway/sirtuin signaling, theoretical concern) · Pregnancy and lactation · Hypersensitivity

Stop if you notice

Severe flushing, chest tightness, or palpitations (slow down or stop)
Any allergic reaction
Pregnancy

Side effects

Flushing, warmth, transient chest tightness, nausea if injected fast; all reduced vs unbuffered NAD+ but not eliminated.

Drug interactions

No major small-molecule interactions. Stimulant effect may compound caffeine/other stimulants.

Labs

None compound-specific required. General metabolic panel if part of a larger protocol.

What these labs mean →

Warnings

Injection-rate-dependent flushing/chest tightness, go slow even though it's buffered. Can transiently raise heart rate / drop blood pressure if rushed. Quality varies, request a COA.

Patient education

Same as NAD+ but easier on injection, still inject slowly. Daytime dosing; it can be energizing. Effects build over weeks; it's not an instant lift. See the NAD+ page for full background.

These apply on top of the universal safety rules.

Pairs well with

MOTS-c / Humanin

mitochondrial

MOTS-c Humanin

SS-31

mito protection

SS-31 (Elamipretide)

Epithalon

longevity

Epithalon

Glutathione

Avoid with

Fast/bolus injection

flush, chest tightness

Active malignancy

sirtuin/repair signaling, theoretical

Pregnancy

Can NAD Buffer + MOTS-c share a syringe?

Give it its own pin. Goes with NAD+, not into a peptide mix.

What it does

NAD Buffer is NAD+ (nicotinamide adenine dinucleotide) formulated at a more physiological pH. NAD+ is the central coenzyme of cellular energy and sirtuin/DNA-repair pathways; buffering reduces the acidity-driven injection discomfort and flushing without changing the active molecule. Same uses as NAD+, energy, recovery, longevity; with better tolerability. See the NAD+ page for full background.

References

researchdosing peptidedosages agelessrx PubMed

From the guide, page 69.